Hotspot

rpoB-trnC-GCA

LSC · 25795-26924 · 1130 bp

Back to Laportea

Pi 0.101

MarkerSeek score 83.80

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1130
variable_sites: 302
pi: 0.101
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGAGGTTCCCAGGGAATTCA	CCGGATTTGAACTGGGGAAA	50.0	50.0	57.9	58.1	1432-1580 bp	0.9	86.9
rpoB-trnC-GCA_p2	CGAGCCCATATCCTTGCTTT	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.0	58.1	1605-1753 bp	0.8	83.1
rpoB-trnC-GCA_p3	CGAGCCCATATCCTTGCTTT	TTTGCCCTATGACCCAATCG	50.0	50.0	58.0	57.9	2253-2271 bp	0.3	59.7
rpoB-trnC-GCA_p4	AGAGGTTCCCAGGGAATTCA	TTTGCCCTATGACCCAATCG	50.0	50.0	57.9	57.9	2080-2095 bp	0.2	51.0
rpoB-trnC-GCA_p5	GCTCTCCGCTTACACAAGAA	TTTGCCCTATGACCCAATCG	50.0	50.0	57.9	57.9	2450-2465 bp	0.2	50.3