Hotspot

trnS-GCU-trnG-UCC

LSC · 7609-8224 · 616 bp

Back to Laportea

Pi 0.108

MarkerSeek score 86.30

Primer available yes

Label trnS-GCU-trnG-UCC

Feature Metadata

label_name: trnS-GCU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 616
variable_sites: 168
pi: 0.108
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 86.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnG-UCC_p1	CAATCCGACGCTTTAGTCCA	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	655-727 bp	1.0	63.7
trnS-GCU-trnG-UCC_p2	AATCCGACGCTTTAGTCCAC	ACGAATCACACTTTTACCACT	50.0	38.1	58.0	55.5	654-726 bp	1.0	63.7
trnS-GCU-trnG-UCC_p3	CAATCCGACGCTTTAGTCCA	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	657-729 bp	1.0	62.6
trnS-GCU-trnG-UCC_p4	AATCCGACGCTTTAGTCCAC	GAACGAATCACACTTTTACCACT	50.0	39.1	58.0	57.3	656-728 bp	1.0	62.6
trnS-GCU-trnG-UCC_p5	ACGGAAAGAGAGGGATTCGA	ACGAATCACACTTTTACCACT	50.0	38.1	58.2	55.5	709-781 bp	1.0	62.5