Hotspot

rps16-trnQ-UUG

LSC · 5683-6107 · 425 bp

Back to Laportea

Pi 0.228

MarkerSeek score 75.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 425
variable_sites: 199
pi: 0.228
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	CGTTGCTTTCTACCACATCG	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.2	58.1	490-739 bp	0.8	77.1
rps16-trnQ-UUG_p2	GCACGTTGCTTTCTACCACA	GAGGTTCGAATCCTTCCGTC	50.0	55.0	59.1	58.1	493-742 bp	0.8	74.9
rps16-trnQ-UUG_p3	CGTTGCTTTCTACCACATCG	TTCGGAGGTTCGAATCCTTC	50.0	50.0	57.2	57.3	494-743 bp	0.8	72.4
rps16-trnQ-UUG_p4	CACGTTGCTTTCTACCACATC	GAGGTTCGAATCCTTCCGTC	47.6	55.0	57.5	58.1	492-741 bp	0.8	71.7
rps16-trnQ-UUG_p5	CACGTTGCTTTCTACCACAT	GAGGTTCGAATCCTTCCGTC	45.0	55.0	56.4	58.1	492-741 bp	0.8	70.3