Hotspot

trnK-UUU-rps16

LSC · 4176-4682 · 507 bp

Back to Laportea

Pi 0.161

MarkerSeek score 79.10

Primer available yes

Label trnK-UUU-rps16

Feature Metadata

label_name: trnK-UUU-rps16
feature_type: igs
region: LSC
length_bp: 507
variable_sites: 211
pi: 0.161
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 79.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	CGACATGCTGCTTTTTCCAG	CGTTGCAATTGATGTTCGCT	50.0	45.0	58.0	58.0	875-1110 bp	0.5	71.5
trnK-UUU-rps16_p2	CGACATGCTGCTTTTTCCAG	ATTTCAACGAAGGCAGGTGT	50.0	45.0	58.0	58.0	724-959 bp	0.4	67.6
trnK-UUU-rps16_p3	TGCTCGTGGTAAAAGCCTTT	ATTTCAACGAAGGCAGGTGT	45.0	45.0	58.0	58.0	1009-1151 bp	0.2	54.4
trnK-UUU-rps16_p4	TGCTCGTGGTAAAAGCCTTT	CGTTGCAATTGATGTTCGCT	45.0	45.0	58.0	58.0	1160-1302 bp	0.2	54.0
trnK-UUU-rps16_p5	TGCTCGTGGTAAAAGCCTTT	TCGTTGCAATTGATGTTCGC	45.0	45.0	58.0	58.0	1161-1303 bp	0.2	54.0