Hotspot

ycf3

LSC · 42592-44546 · 1955 bp

Back to Lancea

Pi 0.00261
MarkerSeek score 61.30
Primer available yes
Label ycf3

Feature Metadata

label_name
ycf3
feature_type
gene
region
LSC
length_bp
1955
variable_sites
5
pi
0.00261
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3_p1 AGTTGACTGATGAGGCCAAA TCCCACGAGAGAGAATGAGA 45.0 50.0 57.0 57.2 2070-2110 bp 1.0 67.6
ycf3_p2 AGTTGACTGATGAGGCCAAAA TCCCACGAGAGAGAATGAGA 42.9 50.0 57.8 57.2 2070-2110 bp 1.0 65.4
ycf3_p3 AGTTGACTGATGAGGCCAAA TCCCACGAGAGAGAATGAGAA 45.0 47.6 57.0 57.9 2070-2110 bp 1.0 65.4
ycf3_p4 AGTTGACTGATGAGGCCAAA TTCCCACGAGAGAGAATGAGA 45.0 47.6 57.0 57.9 2071-2111 bp 1.0 65.4
ycf3_p5 AGTTGACTGATGAGGCCAAAA TCCCACGAGAGAGAATGAGAA 42.9 47.6 57.8 57.9 2070-2110 bp 1.0 63.2