Hotspot

rpl2

IRb · 84696-86188 · 1493 bp

Pi 0.00809

MarkerSeek score 52.00

Primer available yes

Label rpl2

Feature Metadata

label_name: rpl2
feature_type: gene
region: IRb
length_bp: 1493
variable_sites: 12
pi: 0.00809
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl2_p1	AGACGAAGAAAGAATATTTTCTCGC	CGCTTCAACCGGGTTATTCT	36.0	50.0	58.0	58.3	1576-1586 bp	1.0	41.1
rpl2_p2	AGACGAAGAAAGAATATTTTCTCGC	ATCATTACGCTTCAACCGGG	36.0	50.0	58.0	58.3	1583-1593 bp	1.0	41.1
rpl2_p3	GACGAAGAAAGAATATTTTCTCGC	CGCTTCAACCGGGTTATTCT	37.5	50.0	56.8	58.3	1575-1585 bp	1.0	41.1
rpl2_p4	AGACGAAGAAAGAATATTTTCTCGC	ACGCTTCAACCGGGTTATTC	36.0	50.0	58.0	58.6	1577-1587 bp	1.0	41.1
rpl2_p5	ACGAAGAAAGAATATTTTCTCGC	CGCTTCAACCGGGTTATTCT	34.8	50.0	55.7	58.3	1574-1584 bp	1.0	41.1