Hotspot

rps4-trnF-GAA

LSC · 46418-48317 · 1900 bp

Back to Lancea

Pi 0.0304
MarkerSeek score 74.10
Primer available yes
Label rps4-trnF-GAA

Feature Metadata

label_name
rps4-trnF-GAA
feature_type
igs
region
LSC
length_bp
1900
variable_sites
57
pi
0.0304
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
74.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps4-trnF-GAA_p1 GTTAGTCCTGGTAAAGCCCC TCTACCAGCTGAGCTATCCC 55.0 55.0 57.6 58.0 1988-2190 bp 1.0 79.8
rps4-trnF-GAA_p2 GTTAGTCCTGGTAAAGCCCC TTTGAACTGGTGACACGAGG 55.0 50.0 57.6 58.1 2024-2226 bp 1.0 79.4
rps4-trnF-GAA_p3 GTTAGTCCTGGTAAAGCCCC TTAACCATGTGCCAGGAACC 55.0 50.0 57.6 58.1 2047-2249 bp 1.0 79.1
rps4-trnF-GAA_p4 GTTAGTCCTGGTAAAGCCCC GTGCCAGGAACCAGATTTGA 55.0 50.0 57.6 58.1 2039-2241 bp 1.0 79.0
rps4-trnF-GAA_p5 GTTAGTCCTGGTAAAGCCCC TCAGTCCTCTGCTCTACCAG 55.0 55.0 57.6 57.9 2000-2202 bp 1.0 78.7