Hotspot

rps2-rpoC2

LSC · 16120-16351 · 232 bp

Back to Lancea

Pi 0.039
MarkerSeek score 75.30
Primer available yes
Label rps2-rpoC2

Feature Metadata

label_name
rps2-rpoC2
feature_type
igs
region
LSC
length_bp
232
variable_sites
9
pi
0.039
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps2-rpoC2_p1 CCTCCAGCATCTCTTCCAAG TTCGATGGGGAAATGAGAGA 55.0 45.0 57.7 55.9 394-395 bp 1.0 62.9
rps2-rpoC2_p2 AAATGAACTCCTGCCTCCAG TTCGATGGGGAAATGAGAGA 50.0 45.0 57.5 55.9 407-408 bp 1.0 61.4
rps2-rpoC2_p3 CCTCCAGCATCTCTTCCAAG TCGATGGGGAAATGAGAGAT 55.0 45.0 57.7 55.7 393-394 bp 1.0 61.0
rps2-rpoC2_p4 CCATGGCCAAAATGAACTCC TTCGATGGGGAAATGAGAGA 50.0 45.0 57.3 55.9 416-417 bp 1.0 60.0
rps2-rpoC2_p5 GCCAAAATGAACTCCTGCCT TTCGATGGGGAAATGAGAGA 50.0 45.0 58.7 55.9 411-412 bp 1.0 59.5