primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-matK_p1	psbA-matK	1	AAGACGGTTTTCGGTGCTAG	CCCAAAAGCCTCTTCCACTT	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.209070	1426	1956	683-718	1.000000	82.3
psbA-matK_p2	psbA-matK	2	AAGACGGTTTTCGGTGCTAG	TTCCCAAAAGCCTCTTCCAC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.212618	1426	1956	685-720	1.000000	82.3
psbA-matK_p3	psbA-matK	3	AAGACGGTTTTCGGTGCTAG	TTGACCTTCCCAAAAGCCTC	20	20	50.0	50.0	58.2	58.0	0.0	0.0	0.212618	1426	1956	691-726	1.000000	82.3
psbA-matK_p4	psbA-matK	4	AAAGACGGTTTTCGGTGCTA	CCCAAAAGCCTCTTCCACTT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.244678	1426	1956	684-719	1.000000	82.0
psbA-matK_p5	psbA-matK	5	TAAAGACGGTTTTCGGTGCT	CCCAAAAGCCTCTTCCACTT	20	20	45.0	50.0	57.8	58.0	0.0	0.0	0.244678	1426	1956	685-720	1.000000	82.0
matK-rps16_p1	matK-rps16	1	CCACGATCATGAGCAAATGC	GTGCTCAACCCACAGGAAC	20	19	50.0	57.9	57.9	58.7	12.1	0.0	1.784083	3493	4513	1216-1217	1.000000	66.6
matK-rps16_p2	matK-rps16	2	ACAACCACGATCATGAGCAA	GTGCTCAACCCACAGGAAC	20	19	45.0	57.9	57.8	58.7	12.1	0.0	1.852213	3493	4513	1220-1221	1.000000	66.1
matK-rps16_p3	matK-rps16	3	CCACGATCATGAGCAAATGC	TGCTCAACCCACAGGAACT	20	19	50.0	52.6	57.9	58.8	12.1	0.0	1.884684	3493	4513	1215-1216	1.000000	65.8
matK-rps16_p4	matK-rps16	4	ACAACCACGATCATGAGCAA	TGCTCAACCCACAGGAACT	20	19	45.0	52.6	57.8	58.8	12.1	0.0	1.952814	3493	4513	1219-1220	1.000000	65.3
matK-rps16_p5	matK-rps16	5	CCACGATCATGAGCAAATGC	TCCTTGAAAAAGGTGCTCAAC	20	21	50.0	42.9	57.9	56.9	12.1	4.5	2.190869	3493	4513	1228-1229	1.000000	63.4
psbI-trnS-GCT_p1	psbI-trnS-GCT	1	ATCTAATGATCCGGGGCGTA	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	7836	7953	231-231	1.000000	80.1
psbI-trnS-GCT_p2	psbI-trnS-GCT	2	TATCTAATGATCCGGGGCGT	TGGACTAAAGCGTCGGATTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.071561	7836	7953	232-232	1.000000	80.1
psbI-trnS-GCT_p3	psbI-trnS-GCT	3	ATCTAATGATCCGGGGCGTA	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.072949	7836	7953	232-232	1.000000	80.1
psbI-trnS-GCT_p4	psbI-trnS-GCT	4	TATCTAATGATCCGGGGCGT	GTGGACTAAAGCGTCGGATT	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.072949	7836	7953	233-233	1.000000	80.1
psbI-trnS-GCT_p5	psbI-trnS-GCT	5	ATCTAATGATCCGGGGCGTA	ATTGGGAGAGATGGCTGAGT	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.176426	7836	7953	250-250	1.000000	79.2
trnS-GCT-trnR-TCT_p1	trnS-GCT-trnR-TCT	1	CAATCCGACGCTTTAGTCCA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.056187	8042	9758	1788-1789	1.000000	64.3
trnS-GCT-trnR-TCT_p2	trnS-GCT-trnR-TCT	2	AATCCGACGCTTTAGTCCAC	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.0	56.9	0.0	0.0	2.057575	8042	9758	1787-1788	1.000000	64.3
trnS-GCT-trnR-TCT_p3	trnS-GCT-trnR-TCT	3	CAATCCGACGCTTTAGTCCA	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.139515	8042	9758	1794-1795	1.000000	63.6
trnS-GCT-trnR-TCT_p4	trnS-GCT-trnR-TCT	4	AATCCGACGCTTTAGTCCAC	AGGTTTAGAAGACCTCTGTCCT	20	22	50.0	45.5	58.0	57.9	0.0	0.0	2.140903	8042	9758	1793-1794	1.000000	63.6
trnS-GCT-trnR-TCT_p5	trnS-GCT-trnR-TCT	5	ACGGAAAGAGAGGGATTCGA	AGAAGACCTCTGTCCTATCCA	20	21	50.0	47.6	58.2	56.9	0.0	0.0	2.202953	8042	9758	1842-1843	1.000000	63.1
rps2-rpoC2_p1	rps2-rpoC2	1	CCTCCAGCATCTCTTCCAAG	TTCGATGGGGAAATGAGAGA	20	20	55.0	45.0	57.7	55.9	0.0	0.0	2.394244	16120	16351	394-395	1.000000	62.9
rps2-rpoC2_p2	rps2-rpoC2	2	AAATGAACTCCTGCCTCCAG	TTCGATGGGGAAATGAGAGA	20	20	50.0	45.0	57.5	55.9	0.0	0.0	2.569320	16120	16351	407-408	1.000000	61.4
rps2-rpoC2_p3	rps2-rpoC2	3	CCTCCAGCATCTCTTCCAAG	TCGATGGGGAAATGAGAGAT	20	20	55.0	45.0	57.7	55.7	0.0	0.0	2.631507	16120	16351	393-394	1.000000	61.0
rps2-rpoC2_p4	rps2-rpoC2	4	CCATGGCCAAAATGAACTCC	TTCGATGGGGAAATGAGAGA	20	20	50.0	45.0	57.3	55.9	11.0	0.0	2.743896	16120	16351	416-417	1.000000	60.0
rps2-rpoC2_p5	rps2-rpoC2	5	GCCAAAATGAACTCCTGCCT	TTCGATGGGGAAATGAGAGA	20	20	50.0	45.0	58.7	55.9	0.0	0.0	2.804881	16120	16351	411-412	1.000000	59.5
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	CCGGTACATCGTTGTGGATT	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.0	58.1	9.4	0.0	0.077217	36182	36484	426-430	1.000000	80.5
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CCGGTACATCGTTGTGGATT	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.0	58.1	9.4	0.0	0.077217	36182	36484	427-431	1.000000	80.5
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	TTTCCGGTACATCGTTGTGG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	58.2	58.1	9.4	0.0	0.260392	36182	36484	429-433	1.000000	79.0
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	TTTCCGGTACATCGTTGTGG	GAATCGAACCCGCATCTTCT	20	20	50.0	50.0	58.2	58.1	9.4	0.0	0.260392	36182	36484	430-434	1.000000	79.0
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TTGCTTCTCCTGATGGTTGG	AATCGAACCCGCATCTTCTC	20	20	50.0	50.0	57.8	58.1	0.0	0.0	0.263697	36182	36484	471-475	1.000000	78.9
ycf3_p1	ycf3	1	AGTTGACTGATGAGGCCAAA	TCCCACGAGAGAGAATGAGA	20	20	45.0	50.0	57.0	57.2	5.0	0.0	1.766308	42592	44546	2070-2110	1.000000	67.6
ycf3_p2	ycf3	2	AGTTGACTGATGAGGCCAAAA	TCCCACGAGAGAGAATGAGA	21	20	42.9	50.0	57.8	57.2	5.0	0.0	2.037094	42592	44546	2070-2110	1.000000	65.4
ycf3_p3	ycf3	3	AGTTGACTGATGAGGCCAAA	TCCCACGAGAGAGAATGAGAA	20	21	45.0	47.6	57.0	57.9	5.0	0.0	2.044772	42592	44546	2070-2110	1.000000	65.4
ycf3_p4	ycf3	4	AGTTGACTGATGAGGCCAAA	TTCCCACGAGAGAGAATGAGA	20	21	45.0	47.6	57.0	57.9	5.0	0.0	2.044772	42592	44546	2071-2111	1.000000	65.4
ycf3_p5	ycf3	5	AGTTGACTGATGAGGCCAAAA	TCCCACGAGAGAGAATGAGAA	21	21	42.9	47.6	57.8	57.9	5.0	0.0	2.315558	42592	44546	2070-2110	1.000000	63.2
rps4-trnF-GAA_p1	rps4-trnF-GAA	1	GTTAGTCCTGGTAAAGCCCC	TCTACCAGCTGAGCTATCCC	20	20	55.0	55.0	57.6	58.0	0.0	17.4	0.417499	46418	48317	1988-2190	1.000000	79.8
rps4-trnF-GAA_p2	rps4-trnF-GAA	2	GTTAGTCCTGGTAAAGCCCC	TTTGAACTGGTGACACGAGG	20	20	55.0	50.0	57.6	58.1	0.0	20.1	0.466889	46418	48317	2024-2226	1.000000	79.4
rps4-trnF-GAA_p3	rps4-trnF-GAA	3	GTTAGTCCTGGTAAAGCCCC	TTAACCATGTGCCAGGAACC	20	20	55.0	50.0	57.6	58.1	0.0	0.0	0.497122	46418	48317	2047-2249	1.000000	79.1
rps4-trnF-GAA_p4	rps4-trnF-GAA	4	GTTAGTCCTGGTAAAGCCCC	GTGCCAGGAACCAGATTTGA	20	20	55.0	50.0	57.6	58.1	0.0	0.0	0.505294	46418	48317	2039-2241	1.000000	79.0
rps4-trnF-GAA_p5	rps4-trnF-GAA	5	GTTAGTCCTGGTAAAGCCCC	TCAGTCCTCTGCTCTACCAG	20	20	55.0	55.0	57.6	57.9	0.0	0.0	0.551292	46418	48317	2000-2202	1.000000	78.7
ycf4-cemA_p1	ycf4-cemA	1	TTGGCCTATTTCTTGCGTGT	GAGAGAGAAATCCACCAGGG	20	20	45.0	55.0	58.1	57.0	3.3	0.0	1.094029	61034	61940	992-1023	1.000000	73.9
ycf4-cemA_p2	ycf4-cemA	2	GAACAAAAAGCCGCGGAATT	GAGAGAGAAATCCACCAGGG	20	20	45.0	55.0	58.2	57.0	33.5	0.0	1.219981	61034	61940	1010-1041	1.000000	72.9
ycf4-cemA_p3	ycf4-cemA	3	AACAAAAAGCCGCGGAATTG	GAGAGAGAAATCCACCAGGG	20	20	45.0	55.0	58.5	57.0	33.5	0.0	1.501382	61034	61940	1009-1040	1.000000	70.6
ycf4-cemA_p4	ycf4-cemA	4	AATTGAACAAAAAGCCGCGG	GAGAGAGAAATCCACCAGGG	20	20	45.0	55.0	58.5	57.0	24.0	0.0	1.501382	61034	61940	1014-1045	1.000000	70.6
ycf4-cemA_p5	ycf4-cemA	5	CGCGGAATTGGCCTATTTCT	GAGAGAGAAATCCACCAGGG	20	20	50.0	55.0	58.7	57.0	13.8	0.0	1.689318	61034	61940	999-1030	1.000000	69.2
rpl20-rps12_p1	rpl20-rps12	1	TCCGAGCTATATATCCGCGT	ATGTGACAAAATCCCCTGCA	20	20	50.0	45.0	58.0	57.7	4.9	0.0	0.341600	69563	70368	905-910	1.000000	78.9
rpl20-rps12_p2	rpl20-rps12	2	CAGTCTTGAATGAGCCCCTC	ATGTGACAAAATCCCCTGCA	20	20	55.0	45.0	58.0	57.7	0.0	0.0	0.343026	69563	70368	964-969	1.000000	78.8
rpl20-rps12_p3	rpl20-rps12	3	TCCGAGCTATATATCCGCGT	GTGACAAAATCCCCTGCACT	20	20	50.0	50.0	58.0	58.4	4.9	0.0	0.413282	69563	70368	903-908	1.000000	78.4
rpl20-rps12_p4	rpl20-rps12	4	TCCGAGCTATATATCCGCGT	TGACAAAATCCCCTGCACTT	20	20	50.0	45.0	58.0	57.6	4.9	0.0	0.414168	69563	70368	902-907	1.000000	78.4
rpl20-rps12_p5	rpl20-rps12	5	CAGTCTTGAATGAGCCCCTC	GTGACAAAATCCCCTGCACT	20	20	55.0	50.0	58.0	58.4	0.0	0.0	0.414708	69563	70368	962-967	1.000000	78.3
petB_p1	petB	1	TTGGTAGTTCGATCGTGGAA	TCCTACTATTCCTCCTCTAAGTGA	20	24	45.0	41.7	56.6	57.2	15.9	0.0	6.244773	75464	76833	1477-1490	1.000000	41.6
petB_p2	petB	2	TTTGGTAGTTCGATCGTGGA	TCCTACTATTCCTCCTCTAAGTGA	20	24	45.0	41.7	56.6	57.2	15.9	0.0	6.244773	75464	76833	1478-1491	1.000000	41.6
petB_p3	petB	3	TGGTAGTTCGATCGTGGAAT	TCCTACTATTCCTCCTCTAAGTGA	20	24	45.0	41.7	56.4	57.2	15.9	0.0	6.474342	75464	76833	1476-1489	1.000000	41.6
petB_p4	petB	4	AGAACTCGGATTTTGGCCTA	TCCTACTATTCCTCCTCTAAGTGA	20	24	45.0	41.7	56.5	57.2	3.3	0.0	6.310646	75464	76833	1504-1517	1.000000	41.5
petB_p5	petB	5	TAGAACTCGGATTTTGGCCT	TCCTACTATTCCTCCTCTAAGTGA	20	24	45.0	41.7	56.5	57.2	3.3	0.0	6.310646	75464	76833	1505-1518	1.000000	41.5
petD_p1	petD	1	AGTTGAAGGGAATTCTCCGA	CCCTAGATACACATGTCGGG	20	20	45.0	55.0	56.2	56.9	0.4	0.0	2.952452	77039	78266	1246-1307	1.000000	60.2
petD_p2	petD	2	AGTTGAAGGGAATTCTCCGA	GCTTTGAAGCGACTATTCCC	20	20	45.0	50.0	56.2	56.9	0.4	0.0	2.959543	77039	78266	1263-1324	1.000000	60.1
petD_p3	petD	3	TGAAGGGAATTCTCCGAAGAG	CCCTAGATACACATGTCGGG	21	20	47.6	55.0	57.1	56.9	0.4	0.0	3.004447	77039	78266	1243-1304	1.000000	59.8
petD_p4	petD	4	TGAAGGGAATTCTCCGAAGAG	GCTTTGAAGCGACTATTCCC	21	20	47.6	50.0	57.1	56.9	0.4	0.0	3.011538	77039	78266	1260-1321	1.000000	59.7
petD_p5	petD	5	AGTTGAAGGGAATTCTCCGAAG	CCCTAGATACACATGTCGGG	22	20	45.5	55.0	58.1	56.9	0.4	0.0	3.165128	77039	78266	1246-1307	1.000000	58.5
rpl36-infA_p1	rpl36-infA	1	ATTACTATAATGCGCCCCCG	TATGATTCAACCAGAGGGCG	20	20	50.0	50.0	57.6	57.4	4.2	0.0	1.016841	80191	80285	232-232	1.000000	72.6
rpl36-infA_p2	rpl36-infA	2	ATTACTATAATGCGCCCCCG	GATTCAACCAGAGGGCGTAT	20	20	50.0	50.0	57.6	57.4	4.2	0.0	1.018653	80191	80285	229-229	1.000000	72.5
rpl36-infA_p3	rpl36-infA	3	ATTACTATAATGCGCCCCCG	ACGGATACTGCCAGGAGATA	20	20	50.0	50.0	57.6	57.3	4.2	0.0	1.074132	80191	80285	275-275	1.000000	72.0
rpl36-infA_p4	rpl36-infA	4	ATTACTATAATGCGCCCCCG	ATACGGATACTGCCAGGAGA	20	20	50.0	50.0	57.6	57.3	4.2	0.0	1.074132	80191	80285	277-277	1.000000	72.0
rpl36-infA_p5	rpl36-infA	5	ATTACTATAATGCGCCCCCG	TACGGATACTGCCAGGAGAT	20	20	50.0	50.0	57.6	57.3	4.2	0.0	1.074132	80191	80285	276-276	1.000000	72.0
rpl16_p1	rpl16	1	AACCTGCGGTTTTGTCTCAT	AGGGGGAAACTTGTTCATTCT	20	21	45.0	42.9	58.0	57.1	7.1	0.0	1.922274	81723	83026	1435-1476	1.000000	67.3
rpl16_p2	rpl16	2	CGGTTTTGTCTCATCCCCAA	AGGGGGAAACTTGTTCATTCT	20	21	50.0	42.9	58.1	57.1	0.0	0.0	1.999244	81723	83026	1429-1470	1.000000	66.7
rpl16_p3	rpl16	3	AACCTGCGGTTTTGTCTCAT	AGGGGGAAACTTGTTCATTCTT	20	22	45.0	40.9	58.0	57.8	7.1	0.0	2.217746	81723	83026	1435-1476	1.000000	64.9
rpl16_p4	rpl16	4	TGTCTCATCCCCAAGACTCA	AGGGGGAAACTTGTTCATTCT	20	21	50.0	42.9	57.7	57.1	4.4	0.0	2.229191	81723	83026	1423-1464	1.000000	64.8
rpl16_p5	rpl16	5	CGGTTTTGTCTCATCCCCAA	AGGGGGAAACTTGTTCATTCTT	20	22	50.0	40.9	58.1	57.8	0.0	0.0	2.294716	81723	83026	1429-1470	1.000000	64.3
rpl2_p1	rpl2	1	AGACGAAGAAAGAATATTTTCTCGC	CGCTTCAACCGGGTTATTCT	25	20	36.0	50.0	58.0	58.3	14.7	13.0	5.270846	84696	86188	1576-1586	1.000000	41.1
rpl2_p2	rpl2	2	AGACGAAGAAAGAATATTTTCTCGC	ATCATTACGCTTCAACCGGG	25	20	36.0	50.0	58.0	58.3	14.7	6.3	5.341314	84696	86188	1583-1593	1.000000	41.1
rpl2_p3	rpl2	3	GACGAAGAAAGAATATTTTCTCGC	CGCTTCAACCGGGTTATTCT	24	20	37.5	50.0	56.8	58.3	14.7	13.0	5.502643	84696	86188	1575-1585	1.000000	41.1
rpl2_p4	rpl2	4	AGACGAAGAAAGAATATTTTCTCGC	ACGCTTCAACCGGGTTATTC	25	20	36.0	50.0	58.0	58.6	14.7	13.0	5.559138	84696	86188	1577-1587	1.000000	41.1
rpl2_p5	rpl2	5	ACGAAGAAAGAATATTTTCTCGC	CGCTTCAACCGGGTTATTCT	23	20	34.8	50.0	55.7	58.3	14.7	13.0	5.572199	84696	86188	1574-1584	1.000000	41.1
rps12-trnV-GAC_p1	rps12-trnV-GAC	1	TGGCAACTTTACGTAAGGCA	GATGACTTCCACCACGTCAA	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.421509	98829	100481	1711-1749	1.000000	79.1
rps12-trnV-GAC_p2	rps12-trnV-GAC	2	TGGCAACTTTACGTAAGGCA	AACTGATGACTTCCACCACG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	98829	100481	1715-1753	1.000000	79.1
rps12-trnV-GAC_p3	rps12-trnV-GAC	3	TGGCAACTTTACGTAAGGCA	ATGACTTCCACCACGTCAAG	20	20	45.0	50.0	57.7	57.8	16.5	0.0	0.427523	98829	100481	1710-1748	1.000000	79.1
rps12-trnV-GAC_p4	rps12-trnV-GAC	4	TGGCAACTTTACGTAAGGCA	CTGATGACTTCCACCACGTC	20	20	45.0	55.0	57.7	58.3	16.5	0.0	0.542896	98829	100481	1713-1751	1.000000	78.2
rps12-trnV-GAC_p5	rps12-trnV-GAC	5	TGGCAACTTTACGTAAGGCA	GTCAAGGTGACACTCTACCG	20	20	45.0	55.0	57.7	57.7	16.5	5.4	0.606988	98829	100481	1696-1734	1.000000	77.7
trnV-GAC-rps7_p1	trnV-GAC-rps7	1	GATGACTTCCACCACGTCAA	TTCTTCTGCAGTACCTCGAC	20	20	50.0	50.0	57.8	57.0	0.0	14.7	1.175512	138153	140652	2539-2577	1.000000	72.3
trnV-GAC-rps7_p2	trnV-GAC-rps7	2	AACTGATGACTTCCACCACG	TTCTTCTGCAGTACCTCGAC	20	20	50.0	50.0	57.8	57.0	0.0	14.7	1.181527	138153	140652	2543-2581	1.000000	72.2
trnV-GAC-rps7_p3	trnV-GAC-rps7	3	ATGACTTCCACCACGTCAAG	TTCTTCTGCAGTACCTCGAC	20	20	50.0	50.0	57.8	57.0	0.0	14.7	1.181527	138153	140652	2538-2576	1.000000	72.2
trnV-GAC-rps7_p4	trnV-GAC-rps7	4	CTGATGACTTCCACCACGTC	TTCTTCTGCAGTACCTCGAC	20	20	55.0	50.0	58.3	57.0	0.0	14.7	1.296900	138153	140652	2541-2579	1.000000	71.3
trnV-GAC-rps7_p5	trnV-GAC-rps7	5	GATGACTTCCACCACGTCAA	TCTTCTGCAGTACCTCGACG	20	20	50.0	55.0	57.8	59.2	0.0	14.7	1.360701	138153	140652	2538-2576	1.000000	70.8