Hotspot

ycf1

SSC · 120694-126156 · 5463 bp

Pi 0.025

MarkerSeek score 58.70

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5463
variable_sites: 360
pi: 0.025
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAAGTTTGCTATCTGCGGA	CCCCTTTTGTTTACCCCGAA	50.0	50.0	58.0	58.0	945-999 bp	1.0	90.7
ycf1_p2	GGAAGTTTGCTATCTGCGGA	ATTGCAACGTCCTGAATGGT	50.0	45.0	58.0	58.1	1206-1266 bp	1.0	89.5
ycf1_p3	GGAAGTTTGCTATCTGCGGA	GGTCTGAGGATTTCCACGAC	50.0	55.0	58.0	58.0	1249 bp	0.5	60.2
ycf1_p4	GGAAGTTTGCTATCTGCGGA	CTGAGGATTTCCACGACTGG	50.0	55.0	58.0	58.0	1246 bp	0.5	60.2
ycf1_p5	GGAAGTTTGCTATCTGCGGA	TTTAACCGTGTGGGGAAGTG	50.0	50.0	58.0	58.0	1029 bp	0.5	60.0