Hotspot

psbA

LSC · 274-1332 · 1059 bp

Pi 0.00401

MarkerSeek score 38.30

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1059
variable_sites: 12
pi: 0.00401
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	GATCCACTTGGCTACATCCG	GAAAGCCGTGTGCAATGAAA	55.0	45.0	58.1	57.9	1628-1674 bp	1.0	81.3
psbA_p2	CAATCCACTGCCTTGATCCA	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	1642-1688 bp	1.0	81.2
psbA_p3	AATCCACTGCCTTGATCCAC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	1641-1687 bp	1.0	81.2
psbA_p4	ACAATCCACTGCCTTGATCC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.9	57.9	1643-1689 bp	1.0	81.2
psbA_p5	GATCCACTTGGCTACATCCG	CTAGTTCCGGGTTCGAATCC	55.0	55.0	58.1	57.8	1534-1578 bp	1.0	80.7