Hotspot

petB

LSC · 73393-74773 · 1381 bp

Back to Lamium

Pi 0.0177
MarkerSeek score 58.70
Primer available yes
Label petB

Feature Metadata

label_name
petB
feature_type
gene
region
LSC
length_bp
1381
variable_sites
60
pi
0.0177
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petB_p1 GGTAAAGTAGCTCCCGGTTG TTCAAGCGAACCAGCCTATC 55.0 50.0 58.0 58.0 1950-1966 bp 1.0 84.1
petB_p2 GTAAAGTAGCTCCCGGTTGG TTCAAGCGAACCAGCCTATC 55.0 50.0 58.0 58.0 1949-1965 bp 1.0 84.1
petB_p3 GGTAAAGTAGCTCCCGGTTG CAAGCGAACCAGCCTATCTT 55.0 50.0 58.0 58.0 1948-1964 bp 1.0 84.0
petB_p4 GTAAAGTAGCTCCCGGTTGG CAAGCGAACCAGCCTATCTT 55.0 50.0 58.0 58.0 1947-1963 bp 1.0 84.0
petB_p5 GTAAAGTAGCTCCCGGTTGG CTTCAAGCGAACCAGCCTAT 55.0 50.0 58.0 58.0 1950-1966 bp 1.0 84.0