Hotspot

ndhG

SSC · 115092-115622 · 531 bp

Pi 0.0112

MarkerSeek score 41.80

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 16
pi: 0.0112
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 41.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	CCGATCCAATAGCTGCTTCA	TGCGGTAATTGCGTTGAGTA	50.0	45.0	57.8	57.9	1299-1573 bp	1.0	86.6
ndhG_p2	ACACATAAGGGCCCTAACCA	TGCGGTAATTGCGTTGAGTA	50.0	45.0	58.3	57.9	1163-1437 bp	0.875	81.1
ndhG_p3	CCGATCCAATAGCTGCTTCA	TGGTGAGGTTTTTGCTTGGT	50.0	45.0	57.8	58.1	1253-1302 bp	0.875	79.2
ndhG_p4	CCGATCCAATAGCTGCTTCA	TGCTTCGGTTGCTTCGATAA	50.0	45.0	57.8	57.9	1132-1181 bp	0.625	67.7
ndhG_p5	CCGATCCAATAGCTGCTTCA	AGTTTGAACCTCTGCTTCGG	50.0	50.0	57.8	58.1	1144-1175 bp	0.5	61.3