Hotspot

ndhF

SSC · 107859-110081 · 2223 bp

Pi 0.019

MarkerSeek score 57.50

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2223
variable_sites: 110
pi: 0.019
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACAGGAACAAGAGGGATCCA	AGGTCGGGTGAATTGGAAAC	50.0	50.0	58.0	58.1	2505-2511 bp	1.0	83.1
ndhF_p2	AAGAGGGATCCACCGAAGAA	AGGTCGGGTGAATTGGAAAC	50.0	50.0	58.0	58.1	2497-2503 bp	1.0	83.1
ndhF_p3	GGGAGAAAGGGTGGAAAGTG	AGGTCGGGTGAATTGGAAAC	55.0	50.0	58.1	58.1	2577-2583 bp	1.0	82.7
ndhF_p4	AACAGCTTCCGAACTGAAGG	AGGTCGGGTGAATTGGAAAC	50.0	50.0	58.1	58.1	2532-2538 bp	1.0	82.5
ndhF_p5	AGGGTGGAAAGTGAGGAAGA	AGGTCGGGTGAATTGGAAAC	50.0	50.0	57.9	58.1	2570-2576 bp	1.0	82.5