Hotspot

ndhE-ndhG

SSC · 114900-115091 · 192 bp

Back to Lamium

Pi 0.066
MarkerSeek score 74.60
Primer available yes
Label ndhE-ndhG

Feature Metadata

label_name
ndhE-ndhG
feature_type
igs
region
SSC
length_bp
192
variable_sites
42
pi
0.066
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhE-ndhG_p1 TACATCCGTCCCAGGGTATC TTCATCTTTGGACCGTTGGG 55.0 50.0 58.0 58.1 1064-1161 bp 1.0 88.6
ndhE-ndhG_p2 TACATCCGTCCCAGGGTATC ACGTCCTGGTATGGGATCAT 55.0 50.0 58.0 57.9 981-1078 bp 0.875 82.6
ndhE-ndhG_p3 TACATCCGTCCCAGGGTATC CTATTTTGTAGCTGCTGCGC 55.0 50.0 58.0 57.9 1168-1265 bp 0.875 82.3
ndhE-ndhG_p4 TACATCCGTCCCAGGGTATC TGCTTCGGTTGCTTCGATAA 55.0 45.0 58.0 57.9 1568-1617 bp 0.625 68.7
ndhE-ndhG_p5 GGACAAGCGGATTCACATCT TGCTTCGGTTGCTTCGATAA 50.0 45.0 58.0 57.9 1641-1690 bp 0.625 68.3