Hotspot

rpl32-trnL(UAG)

SSC · 110747-111142 · 396 bp

Back to Lamium

Pi 0.0349
MarkerSeek score 66.70
Primer available yes
Label rpl32-trnL(UAG)

Feature Metadata

label_name
rpl32-trnL(UAG)
feature_type
igs
region
SSC
length_bp
396
variable_sites
34
pi
0.0349
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL(UAG)_p1 GGGCAGCGTTAAAAGCATTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.9 58.0 525-844 bp 1.0 88.0
rpl32-trnL(UAG)_p2 GGGCAGCGTTAAAAGCATTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.9 58.0 526-845 bp 1.0 88.0
rpl32-trnL(UAG)_p3 GGGCAGCGTTAAAAGCATTT TGATCCACATAAAAGGGCCG 45.0 50.0 57.9 57.9 1128-1451 bp 1.0 87.6
rpl32-trnL(UAG)_p4 GTTTCCAATTCACCCGACCT AGCGTGTCTACCGATTTCAC 50.0 50.0 58.1 58.0 1053-1384 bp 0.875 80.7
rpl32-trnL(UAG)_p5 GTTTCCAATTCACCCGACCT CAGCGTGTCTACCGATTTCA 50.0 50.0 58.1 58.0 1054-1385 bp 0.875 80.7