Hotspot

psbT-psbN

LSC · 72739-72811 · 73 bp

Back to Lamium

Pi 0.0424
MarkerSeek score 66.90
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
73
variable_sites
9
pi
0.0424
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 CCTAAAGTTCCAACTAAAAAGT CGAGGAACACGGGGACTA 31.8 61.1 52.2 58.0 110-116 bp 0.625 31.8
psbT-psbN_p2 CCTAAAGTTCCAACTAAAAAGT CGAGGAACACGGGGACTAG 31.8 63.2 52.2 59.2 110-116 bp 0.625 31.8
psbT-psbN_p3 CCTAAAGTTCCAACTAAAAAGT TTCGAGGAACACGGGGACTA 31.8 55.0 52.2 60.3 112-118 bp 0.625 31.8
psbT-psbN_p4 CCTAAAGTTCCAACTAAAAAGT TCGAGGAACACGGGGACTA 31.8 57.9 52.2 59.6 111-117 bp 0.625 31.8
psbT-psbN_p5 CCTAAAGTTCCAACTAAAAAGT TCGAGGAACACGGGGACTAG 31.8 60.0 52.2 60.7 111-117 bp 0.625 31.8