Hotspot

psbB-psbT

LSC · 72448-72636 · 189 bp

Back to Lamium

Pi 0.0324
MarkerSeek score 73.30
Primer available yes
Label psbB-psbT

Feature Metadata

label_name
psbB-psbT
feature_type
igs
region
LSC
length_bp
189
variable_sites
16
pi
0.0324
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbB-psbT_p1 CAACTACACGAAGACAGGCA CCCTAGAGTCGAGACTAAAAGG 50.0 50.0 57.9 57.1 210-265 bp 1.0 64.6
psbB-psbT_p2 GGGGATCCAACTACACGAAG CCCTAGAGTCGAGACTAAAAGG 55.0 50.0 57.7 57.1 217-272 bp 1.0 63.2
psbB-psbT_p3 TGGGGATCCAACTACACGAA CCCTAGAGTCGAGACTAAAAGG 50.0 50.0 58.4 57.1 218-273 bp 1.0 62.8
psbB-psbT_p4 AACTACACGAAGACAGGCAG CCCTAGAGTCGAGACTAAAAGG 50.0 50.0 57.6 57.1 209-264 bp 1.0 62.3
psbB-psbT_p5 CCAACTACACGAAGACAGGC CCCTAGAGTCGAGACTAAAAGG 55.0 50.0 58.6 57.1 211-266 bp 1.0 61.2