Hotspot

petA-psbJ

LSC · 61931-62970 · 1040 bp

Back to Lamium

Pi 0.0317
MarkerSeek score 77.80
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1040
variable_sites
84
pi
0.0317
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 GAGAAGGTTCAATTGGCCGA TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1067-1111 bp 1.0 84.8
petA-psbJ_p2 TCGAGAAGGTTCAATTGGCC TCCGGATTAGGTTCATCCCT 50.0 50.0 58.2 57.5 1069-1113 bp 1.0 84.8
petA-psbJ_p3 GAGAAGGTTCAATTGGCCGA CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1066-1110 bp 1.0 84.3
petA-psbJ_p4 TCGAGAAGGTTCAATTGGCC CCGGATTAGGTTCATCCCTG 50.0 55.0 58.2 57.4 1068-1112 bp 1.0 84.3
petA-psbJ_p5 AGAAGGTTCAATTGGCCGAA TCCGGATTAGGTTCATCCCT 45.0 50.0 57.7 57.5 1066-1110 bp 1.0 83.9