Hotspot

rbcL-accD

LSC · 55548-56252 · 705 bp

Back to Lamium

Pi 0.0243
MarkerSeek score 67.30
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
705
variable_sites
42
pi
0.0243
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.99
markerseek_score
67.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 TTGCAAGTGGAGTCCTGAAC ACTAGAATTGTCACGACCGC 50.0 50.0 58.0 58.0 979-1010 bp 1.0 85.9
rbcL-accD_p2 TCATGGTATGCACTTCCGTG ACTAGAATTGTCACGACCGC 50.0 50.0 58.0 58.0 1405-1436 bp 1.0 84.7
rbcL-accD_p3 TCTTCACATTCACCGTGCAA ACTAGAATTGTCACGACCGC 45.0 50.0 58.0 58.0 1453-1484 bp 1.0 84.4
rbcL-accD_p4 TTCTTCACATTCACCGTGCA ACTAGAATTGTCACGACCGC 45.0 50.0 58.0 58.0 1454-1485 bp 1.0 84.4
rbcL-accD_p5 TTGAATGCTACTGCGGGTAC ACTAGAATTGTCACGACCGC 50.0 50.0 58.0 58.0 1605-1636 bp 1.0 84.2