Hotspot

psbZ-trnG(GCC)

LSC · 35545-35786 · 242 bp

Back to Lamium

Pi 0.0308
MarkerSeek score 69.00
Primer available yes
Label psbZ-trnG(GCC)

Feature Metadata

label_name
psbZ-trnG(GCC)
feature_type
igs
region
LSC
length_bp
242
variable_sites
18
pi
0.0308
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.93
markerseek_score
69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG(GCC)_p1 GGGTTAGTCTTTCTGGTGGG AATCGAACCCGCATCTTCTC 55.0 50.0 57.5 58.1 327-345 bp 1.0 84.0
psbZ-trnG(GCC)_p2 GGGTTAGTCTTTCTGGTGGG GAATCGAACCCGCATCTTCT 55.0 50.0 57.5 58.1 328-346 bp 1.0 83.9
psbZ-trnG(GCC)_p3 GGGTTAGTCTTTCTGGTGGG GAACCCGCATCTTCTCCTTG 55.0 55.0 57.5 58.6 323-341 bp 1.0 79.5
psbZ-trnG(GCC)_p4 GGGTTAGTCTTTCTGGTGGG TACCATTCGACCATATCCGC 55.0 50.0 57.5 57.3 289-307 bp 1.0 79.1
psbZ-trnG(GCC)_p5 AGTCTTTCTGGTGGGTATCCT AATCGAACCCGCATCTTCTC 47.6 50.0 57.8 58.1 322-340 bp 1.0 78.3