Hotspot

trnE(UUC)-trnT(GGU)

LSC · 30518-31102 · 585 bp

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Pi 0.0219
MarkerSeek score 71.40
Primer available yes
Label trnE(UUC)-trnT(GGU)

Feature Metadata

label_name
trnE(UUC)-trnT(GGU)
feature_type
igs
region
LSC
length_bp
585
variable_sites
33
pi
0.0219
unique_haplotype_count
8
primer_available
yes
alignment_reliability
0.97
markerseek_score
71.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE(UUC)-trnT(GGU)_p1 CATAGTACCCTACCCCCAGG GAACCGATGACTTACGCCTT 60.0 50.0 58.0 58.0 695-784 bp 1.0 87.2
trnE(UUC)-trnT(GGU)_p2 CATAGTACCCTACCCCCAGG CCATGGCGTTACTCTACCAC 60.0 55.0 58.0 58.1 674-763 bp 1.0 86.9
trnE(UUC)-trnT(GGU)_p3 CATAGTACCCTACCCCCAGG TGGCGTTACTCTACCACTGA 60.0 50.0 58.0 58.1 671-760 bp 1.0 86.8
trnE(UUC)-trnT(GGU)_p4 CATAGTACCCTACCCCCAGG CCGATGACTTACGCCTTACC 60.0 55.0 58.0 58.2 692-781 bp 1.0 86.0
trnE(UUC)-trnT(GGU)_p5 GCTGCCTCCTTGAAAGAGAG GAACCGATGACTTACGCCTT 55.0 50.0 58.3 58.0 660-749 bp 1.0 85.6