Hotspot

rps16-trnQ(UUG)

LSC · 6045-7031 · 987 bp

Back to Lamium

Pi 0.0283
MarkerSeek score 68.60
Primer available yes
Label rps16-trnQ(UUG)

Feature Metadata

label_name
rps16-trnQ(UUG)
feature_type
igs
region
LSC
length_bp
987
variable_sites
71
pi
0.0283
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.98
markerseek_score
68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ(UUG)_p1 GTCTTTCAAGTCGCACGTTG GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.0 58.1 1045-1089 bp 1.0 86.7
rps16-trnQ(UUG)_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1038-1082 bp 1.0 85.7
rps16-trnQ(UUG)_p3 ATCGTGTCTTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.9 58.1 1050-1094 bp 1.0 85.6
rps16-trnQ(UUG)_p4 TGTCTTTCAAGTCGCACGTT GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.4 58.1 1046-1090 bp 1.0 83.9
rps16-trnQ(UUG)_p5 GTCTTTCAAGTCGCACGTTG TTCGGAGGTTCGAATCCTTC 50.0 50.0 58.0 57.3 1049-1093 bp 1.0 82.0