Hotspot

trnH(GUG)-psbA

LSC · 78-273 · 196 bp

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Pi 0.0851
MarkerSeek score 84.20
Primer available yes
Label trnH(GUG)-psbA

Feature Metadata

label_name
trnH(GUG)-psbA
feature_type
igs
region
LSC
length_bp
196
variable_sites
40
pi
0.0851
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH(GUG)-psbA_p1 GATCCACTTGGCTACATCCG CAACCGTGCTAACCTTGGTA 55.0 50.0 58.1 57.8 315-348 bp 1.0 87.8
trnH(GUG)-psbA_p2 GATCCACTTGGCTACATCCG ATCAACCGTGCTAACCTTGG 55.0 50.0 58.1 58.2 317-350 bp 1.0 87.7
trnH(GUG)-psbA_p3 CAATCCACTGCCTTGATCCA CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 329-362 bp 1.0 87.6
trnH(GUG)-psbA_p4 AATCCACTGCCTTGATCCAC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 328-361 bp 1.0 87.6
trnH(GUG)-psbA_p5 ACAATCCACTGCCTTGATCC CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 330-363 bp 1.0 87.6