Hotspot

ycf34

Genome · 48390-48674 · 285 bp

Back to Laminaria

Pi 0.0351

MarkerSeek score 58.10

Primer available yes

Label ycf34

Feature Metadata

label_name: ycf34
feature_type: gene
region: Genome
length_bp: 285
variable_sites: 10
pi: 0.0351
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf34_p1	CTGGGTTTGAAGCTGACACT	TGCAAATCGTGAGGGTTCAA	50.0	45.0	58.0	58.0	645-649 bp	1.0	82.2
ycf34_p2	CTGGGTTTGAAGCTGACACT	CCGTTGATGCAAATCGTGAG	50.0	50.0	58.0	57.8	652-656 bp	1.0	81.0
ycf34_p3	CTGGGTTTGAAGCTGACACT	CGTTGATGCAAATCGTGAGG	50.0	50.0	58.0	57.8	651-655 bp	1.0	81.0
ycf34_p4	CTGGGTTTGAAGCTGACACT	TAAGGCCGTGGATTGCAAAT	50.0	45.0	58.0	58.2	1061 bp	1.0	80.8
ycf34_p5	GCCAGCCAGTTAACTAAGCA	TGCAAATCGTGAGGGTTCAA	50.0	45.0	58.2	58.0	982-986 bp	1.0	80.7