Hotspot

ftsH-psbH

Genome · 105240-105340 · 101 bp

Back to Laminaria

Pi 0.0886

MarkerSeek score 68.80

Primer available yes

Label ftsH-psbH

Feature Metadata

label_name: ftsH-psbH
feature_type: igs
region: Genome
length_bp: 101
variable_sites: 7
pi: 0.0886
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.78
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ftsH-psbH_p1	AACGCGAGGCCAATTATCTT	GGCCCAAAAGCCGTATAAGT	45.0	50.0	58.0	58.2	510-927 bp	1.0	80.0
ftsH-psbH_p2	CGCGAGGCCAATTATCTTCT	GGCCCAAAAGCCGTATAAGT	50.0	50.0	58.1	58.2	508-925 bp	1.0	79.3
ftsH-psbH_p3	AGCAACCTCTTTTAACGCGA	GGCCCAAAAGCCGTATAAGT	45.0	50.0	58.1	58.2	523-940 bp	1.0	79.2
ftsH-psbH_p4	TGCTGGTCTAGAGGGAGTTC	GGCCCAAAAGCCGTATAAGT	55.0	50.0	58.1	58.2	696-1113 bp	1.0	78.8
ftsH-psbH_p5	GCTGGTCTAGAGGGAGTTCT	GGCCCAAAAGCCGTATAAGT	55.0	50.0	57.8	58.2	695-1112 bp	1.0	78.8