Hotspot

rps10-psaE

Genome · 102956-103009 · 54 bp

Back to Laminaria

Pi 0.0741

MarkerSeek score 69.50

Primer available yes

Label rps10-psaE

Feature Metadata

label_name: rps10-psaE
feature_type: igs
region: Genome
length_bp: 54
variable_sites: 4
pi: 0.0741
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps10-psaE_p1	GTTATGCCAGGTGATCGTGT	TGCCTCTCCTTAGCATACGT	50.0	50.0	58.0	58.2	861-2808 bp	1.0	78.6
rps10-psaE_p2	TCGAACTATTGGTGCTGGTG	TGCCTCTCCTTAGCATACGT	50.0	50.0	57.9	58.2	769-2716 bp	1.0	78.1
rps10-psaE_p3	TGGTCGAACTATTGGTGCTG	TGCCTCTCCTTAGCATACGT	50.0	50.0	57.9	58.2	772-2719 bp	1.0	78.1
rps10-psaE_p4	GTCGAACTATTGGTGCTGGT	TGCCTCTCCTTAGCATACGT	50.0	50.0	57.9	58.2	770-2717 bp	1.0	78.1
rps10-psaE_p5	GTGGTCGAACTATTGGTGCT	TGCCTCTCCTTAGCATACGT	50.0	50.0	57.9	58.2	773-2720 bp	1.0	78.1