Hotspot

rns-F6D16_pgt019

Genome · 83798-83915 · 118 bp

Back to Laminaria

Pi 0.0254

MarkerSeek score 67.10

Primer available yes

Label rns-F6D16_pgt019

Feature Metadata

label_name: rns-F6D16_pgt019
feature_type: igs
region: Genome
length_bp: 118
variable_sites: 3
pi: 0.0254
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rns-F6D16_pgt019_p1	GACTGGGGTGAAGTCGTAAC	GCCATCCTGGATTTGAACCA	55.0	50.0	57.9	58.1	245 bp	1.0	79.2
rns-F6D16_pgt019_p2	GTAACAAGGTAGCCGTACGG	GCCATCCTGGATTTGAACCA	55.0	50.0	58.1	58.1	230 bp	1.0	79.2
rns-F6D16_pgt019_p3	GACTGGGGTGAAGTCGTAAC	AATGGGCCATCCTGGATTTG	55.0	50.0	57.9	58.2	250 bp	1.0	78.7
rns-F6D16_pgt019_p4	GACTGGGGTGAAGTCGTAAC	CAAATGGGCCATCCTGGATT	55.0	50.0	57.9	58.2	252 bp	1.0	78.7
rns-F6D16_pgt019_p5	AGGTAAGGCTAGTGACTGGG	GCCATCCTGGATTTGAACCA	55.0	50.0	58.1	58.1	258 bp	1.0	78.7