Hotspot

psbA-cbbx

Genome · 74614-75162 · 549 bp

Back to Laminaria

Pi 0.0255

MarkerSeek score 59.30

Primer available yes

Label psbA-cbbx

Feature Metadata

label_name: psbA-cbbx
feature_type: igs
region: Genome
length_bp: 549
variable_sites: 14
pi: 0.0255
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-cbbx_p1	CTGCTCCTTCTGTTGTTGGT	GCTAGTAGTGTATTTAGTTTGTAA	50.0	29.2	58.0	52.4	596-746 bp	1.0	41.8
psbA-cbbx_p2	TGTTGCGATTTCTGCTCCTT	GCTAGTAGTGTATTTAGTTTGTAA	45.0	29.2	58.1	52.4	607-757 bp	1.0	41.8
psbA-cbbx_p3	TCTGCTCCTTCTGTTGTTGG	GCTAGTAGTGTATTTAGTTTGTAA	50.0	29.2	57.7	52.4	597-747 bp	1.0	41.8
psbA-cbbx_p4	TTCCTGTTGCGATTTCTGCT	GCTAGTAGTGTATTTAGTTTGTAA	45.0	29.2	58.1	52.4	611-761 bp	1.0	41.7
psbA-cbbx_p5	CTTCCTGTTGCGATTTCTGC	GCTAGTAGTGTATTTAGTTTGTAA	50.0	29.2	57.7	52.4	612-762 bp	1.0	41.7