Hotspot

F6D16_pgt016-psaC

Genome · 72780-72849 · 70 bp

Back to Laminaria

Pi 0.114
MarkerSeek score 78.20
Primer available yes
Label F6D16_pgt016-psaC

Feature Metadata

label_name
F6D16_pgt016-psaC
feature_type
igs
region
Genome
length_bp
70
variable_sites
8
pi
0.114
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
F6D16_pgt016-psaC_p1 TAATTGGGTCACCTGGGACT ACAGCTTGCCCAACAGATTT 50.0 45.0 57.9 58.3 227 bp 1.0 80.1
F6D16_pgt016-psaC_p2 TAATTGGGTCACCTGGGACT AAACAGCTTGCCCAACAGAT 50.0 45.0 57.9 58.3 229 bp 1.0 80.0
F6D16_pgt016-psaC_p3 TAATTGGGTCACCTGGGACT AACAGCTTGCCCAACAGATT 50.0 45.0 57.9 58.3 228 bp 1.0 80.0
F6D16_pgt016-psaC_p4 TAATTGGGTCACCTGGGACT ATTTAGCCGCTGAAACAACG 50.0 45.0 57.9 57.4 190 bp 1.0 78.0
F6D16_pgt016-psaC_p5 TAATTGGGTCACCTGGGACT CGCGTAGTATGGGATTAGCA 50.0 50.0 57.9 57.3 172 bp 1.0 77.6