Hotspot

rpl33-clpC

Genome · 69575-69880 · 306 bp

Back to Laminaria

Pi 0.0261

MarkerSeek score 53.50

Primer available yes

Label rpl33-clpC

Feature Metadata

label_name: rpl33-clpC
feature_type: igs
region: Genome
length_bp: 306
variable_sites: 8
pi: 0.0261
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-clpC_p1	TTTGGCCAGTTCTCCTTGAC	TACACCACAGCCTTCACCTA	50.0	50.0	58.0	58.0	555 bp	1.0	81.6
rpl33-clpC_p2	TGGCCAGTTCTCCTTGACTA	TACACCACAGCCTTCACCTA	50.0	50.0	58.0	58.0	553 bp	1.0	81.5
rpl33-clpC_p3	TTTGGCCAGTTCTCCTTGAC	AGGGTCCAATTTTGCTCGTT	50.0	45.0	58.0	58.0	1029 bp	1.0	81.1
rpl33-clpC_p4	TGGCCAGTTCTCCTTGACTA	AGGGTCCAATTTTGCTCGTT	50.0	45.0	58.0	58.0	1027 bp	1.0	81.0
rpl33-clpC_p5	TTTGGCCAGTTCTCCTTGAC	TCGGCTACTGCTGTTTTACC	50.0	50.0	58.0	57.9	1142 bp	1.0	80.3