Hotspot

psaF-ORF2

Genome · 62447-62813 · 367 bp

Back to Laminaria

Pi 0.039

MarkerSeek score 63.80

Primer available yes

Label psaF-ORF2

Feature Metadata

label_name: psaF-ORF2
feature_type: igs
region: Genome
length_bp: 367
variable_sites: 14
pi: 0.039
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaF-ORF2_p1	GCCGATGTTGCAGGTTTAAC	CCGTATGGAGCCTCAACTTC	50.0	55.0	58.0	58.1	1383-1386 bp	1.0	81.3
psaF-ORF2_p2	CAGTCTCAGCTTGGCAAGAA	CCGTATGGAGCCTCAACTTC	50.0	55.0	58.1	58.1	971-974 bp	1.0	81.3
psaF-ORF2_p3	GCTACTTCAAACCCAACGGA	CCGTATGGAGCCTCAACTTC	50.0	55.0	58.1	58.1	1053-1056 bp	1.0	81.1
psaF-ORF2_p4	CAGTCTCAGCTTGGCAAGAA	TACCTGCTCGTGCCGATATA	50.0	50.0	58.1	58.1	859-862 bp	1.0	81.0
psaF-ORF2_p5	GCCGATGTTGCAGGTTTAAC	TACCTGCTCGTGCCGATATA	50.0	50.0	58.0	58.1	1271-1274 bp	1.0	80.9