Hotspot

ycf66-rpl19

Genome · 41731-43088 · 1358 bp

Back to Laminaria

Pi 0.0163

MarkerSeek score 68.70

Primer available yes

Label ycf66-rpl19

Feature Metadata

label_name: ycf66-rpl19
feature_type: igs
region: Genome
length_bp: 1358
variable_sites: 22
pi: 0.0163
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf66-rpl19_p1	CATCACGCGATAGTTCTGGT	TGAACACTTTCAACAGAATCA	50.0	33.3	57.8	53.6	1535 bp	1.0	41.6
ycf66-rpl19_p2	CACGCGATAGTTCTGGTCTT	TGAACACTTTCAACAGAATCA	50.0	33.3	57.7	53.6	1532 bp	1.0	41.6
ycf66-rpl19_p3	CATCACGCGATAGTTCTGGT	TGAACACTTTCAACAGAATCAA	50.0	31.8	57.8	54.4	1535 bp	1.0	41.6
ycf66-rpl19_p4	CACGCGATAGTTCTGGTCTT	TGAACACTTTCAACAGAATCAA	50.0	31.8	57.7	54.4	1532 bp	1.0	41.6
ycf66-rpl19_p5	TCATCACGCGATAGTTCTGG	TGAACACTTTCAACAGAATCA	50.0	33.3	57.5	53.6	1536 bp	1.0	41.6