Hotspot

F6D16_pgt008-dnaK

Genome · 35867-36245 · 379 bp

Back to Laminaria

Pi 0.038
MarkerSeek score 69.70
Primer available yes
Label F6D16_pgt008-dnaK

Feature Metadata

label_name
F6D16_pgt008-dnaK
feature_type
igs
region
Genome
length_bp
379
variable_sites
14
pi
0.038
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
F6D16_pgt008-dnaK_p1 GTTGCGCTCTACCACTGAG CTGCTTCTGCTAACCCAGAA 57.9 50.0 58.3 57.5 483-494 bp 1.0 70.2
F6D16_pgt008-dnaK_p2 GTTGCGCTCTACCACTGAG ATTGCTGCTTCTGCTAACCC 57.9 50.0 58.3 58.5 487-498 bp 1.0 69.7
F6D16_pgt008-dnaK_p3 GTTGCGCTCTACCACTGAG TTGCTGCTTCTGCTAACCC 57.9 52.6 58.3 58.1 486-497 bp 1.0 65.6
F6D16_pgt008-dnaK_p4 CAAGGTTGCGCTCTACCAC CTGCTTCTGCTAACCCAGAA 57.9 50.0 58.8 57.5 487-498 bp 1.0 65.5
F6D16_pgt008-dnaK_p5 CAAGGTTGCGCTCTACCAC ATTGCTGCTTCTGCTAACCC 57.9 50.0 58.8 58.5 491-502 bp 1.0 65.1