Hotspot

thiG-F6D16_pgt008

Genome · 35668-35794 · 127 bp

Back to Laminaria

Pi 0.0496
MarkerSeek score 76.40
Primer available yes
Label thiG-F6D16_pgt008

Feature Metadata

label_name
thiG-F6D16_pgt008
feature_type
igs
region
Genome
length_bp
127
variable_sites
6
pi
0.0496
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.95
markerseek_score
76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
thiG-F6D16_pgt008_p1 CCAGTTCCAACAATAAGGCG TTTGCGAGTGTAGCTCAGTG 50.0 50.0 57.1 58.2 264-270 bp 1.0 74.5
thiG-F6D16_pgt008_p2 TCCCAGTTCCAACAATAAGGC TTTGCGAGTGTAGCTCAGTG 47.6 50.0 58.2 58.2 266-272 bp 1.0 72.3
thiG-F6D16_pgt008_p3 CCCAGTTCCAACAATAAGGC TTTGCGAGTGTAGCTCAGTG 50.0 50.0 56.7 58.2 265-271 bp 1.0 71.2
thiG-F6D16_pgt008_p4 CCAGTTCCAACAATAAGGCG TGATTTGCGAGTGTAGCTCA 50.0 45.0 57.1 57.3 267-273 bp 1.0 70.3
thiG-F6D16_pgt008_p5 CCAGTTCCAACAATAAGGCG ATTTGCGAGTGTAGCTCAGT 50.0 45.0 57.1 57.2 265-271 bp 1.0 70.2