Hotspot

F6D16_pgt005-F6D16_pgt006

Genome · 25339-25385 · 47 bp

Back to Laminaria

Pi 0.0426

MarkerSeek score 70.90

Primer available yes

Label F6D16_pgt005-F6D16_pgt006

Feature Metadata

label_name: F6D16_pgt005-F6D16_pgt006
feature_type: igs
region: Genome
length_bp: 47
variable_sites: 2
pi: 0.0426
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 70.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
F6D16_pgt005-F6D16_pgt006_p1	CTGCGACTTTCGGGTTATGA	CGCAGTTTGGTAGCGTATCT	50.0	50.0	58.0	58.1	164 bp	1.0	80.3
F6D16_pgt005-F6D16_pgt006_p2	TGCGACTTTCGGGTTATGAG	CGCAGTTTGGTAGCGTATCT	50.0	50.0	58.0	58.1	163 bp	1.0	80.3
F6D16_pgt005-F6D16_pgt006_p3	CTACCAAACTGCTCCACTCC	CGCAGTTTGGTAGCGTATCT	55.0	50.0	57.9	58.1	134 bp	1.0	79.8
F6D16_pgt005-F6D16_pgt006_p4	CTTTCGGGTTATGAGCCCAA	CGCAGTTTGGTAGCGTATCT	50.0	50.0	57.9	58.1	158 bp	1.0	79.4
F6D16_pgt005-F6D16_pgt006_p5	TTTCGGGTTATGAGCCCAAC	CGCAGTTTGGTAGCGTATCT	50.0	50.0	58.2	58.1	157 bp	1.0	79.0