Hotspot

rpoC2-F6D16_pgt004

Genome · 18690-24501 · 5812 bp

Back to Laminaria

Pi 0.0141

MarkerSeek score 52.70

Primer available yes

Label rpoC2-F6D16_pgt004

Feature Metadata

label_name: rpoC2-F6D16_pgt004
feature_type: igs
region: Genome
length_bp: 5812
variable_sites: 82
pi: 0.0141
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2-F6D16_pgt004_p1	CCGTTTTTCACCGTTTCGTT	AGCATCTAATGTGGGAGAGGA	45.0	47.6	57.8	57.9	543 bp	0.5	50.2
rpoC2-F6D16_pgt004_p2	CCGTTTTTCACCGTTTCGTT	AATGTGGGAGAGGAGAAGCC	45.0	55.0	57.8	59.1	536 bp	0.5	49.9
rpoC2-F6D16_pgt004_p3	GCTCCTCTAGAGAAAAGCCG	AGCATCTAATGTGGGAGAGGA	55.0	47.6	57.8	57.9	854 bp	0.5	49.8
rpoC2-F6D16_pgt004_p4	GAGCCGTTTTTCACCGTTTC	AGCATCTAATGTGGGAGAGGA	50.0	47.6	58.2	57.9	546 bp	0.5	49.6
rpoC2-F6D16_pgt004_p5	GCTCCTCTAGAGAAAAGCCG	AATGTGGGAGAGGAGAAGCC	55.0	55.0	57.8	59.1	847 bp	0.5	49.6