Hotspot

ndhD

SSC · 114545-116074 · 1530 bp

Back to Lagotis

Pi 0.00719

MarkerSeek score 48.20

Primer available yes

Label ndhD

Feature Metadata

label_name: ndhD
feature_type: gene
region: SSC
length_bp: 1530
variable_sites: 16
pi: 0.00719
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD_p1	TGGGTCCCTTTTCTTTGGTG	CCCTGGGATGGATGTAAAGC	50.0	55.0	57.9	58.0	2436-2439 bp	1.0	80.8
ndhD_p2	TTGGAGTTGGGACCCAAAAG	CCCTGGGATGGATGTAAAGC	50.0	55.0	57.9	58.0	2223-2226 bp	1.0	80.8
ndhD_p3	CTAACGAAGCATGGGGATCC	CCCTGGGATGGATGTAAAGC	55.0	55.0	58.1	58.0	2245-2248 bp	1.0	80.5
ndhD_p4	TTGGTGTGAACTGGCAAGAA	CCCTGGGATGGATGTAAAGC	45.0	55.0	57.9	58.0	1939-1942 bp	1.0	80.2
ndhD_p5	TTTGGTGTGAACTGGCAAGA	CCCTGGGATGGATGTAAAGC	45.0	55.0	57.9	58.0	1940-1943 bp	1.0	80.2