Hotspot

rps15-ycf1

SSC · 122396-122809 · 414 bp

Back to Lagotis

Pi 0.0223

MarkerSeek score 59.40

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 414
variable_sites: 13
pi: 0.0223
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 59.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	CAGCAGTCGTTGACGTTTTC	GCCGGTTGGGAAGAGTTTAA	50.0	50.0	58.0	58.1	1132-1139 bp	1.0	83.8
rps15-ycf1_p2	TCCCAACGCGTTACATAAGG	GCCGGTTGGGAAGAGTTTAA	50.0	50.0	58.0	58.1	1523-1530 bp	1.0	82.9
rps15-ycf1_p3	CAGCAGTCGTTGACGTTTTC	CCCATCCGTTTGTCTGGAAA	50.0	50.0	58.0	58.1	1071-1074 bp	0.666667	69.6
rps15-ycf1_p4	AGTCGTTGACGTTTTCCCAA	CCCATCCGTTTGTCTGGAAA	45.0	50.0	58.0	58.1	1067-1070 bp	0.666667	69.4
rps15-ycf1_p5	TCCCAACGCGTTACATAAGG	CCCATCCGTTTGTCTGGAAA	50.0	50.0	58.0	58.1	1462-1465 bp	0.666667	68.7