Hotspot

trnL-UAG-ccsA

SSC · 113218-113349 · 132 bp

Back to Lagotis

Pi 0.0504

MarkerSeek score 83.80

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 132
variable_sites: 9
pi: 0.0504
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 83.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	TAGACACGCTGCTCTTAGGA	AGAATTGAAACGATCGAAAAAGA	50.0	30.4	57.9	55.2	248-255 bp	1.0	46.9
trnL-UAG-ccsA_p2	AAATTGGTAGACACGCTGCT	AGAATTGAAACGATCGAAAAAGA	45.0	30.4	57.8	55.2	255-262 bp	1.0	46.8
trnL-UAG-ccsA_p3	GGTAGACACGCTGCTCTTAG	AGAATTGAAACGATCGAAAAAGA	55.0	30.4	57.8	55.2	250-257 bp	1.0	46.8
trnL-UAG-ccsA_p4	GTAGACACGCTGCTCTTAGG	AGAATTGAAACGATCGAAAAAGA	55.0	30.4	57.8	55.2	249-256 bp	1.0	46.8
trnL-UAG-ccsA_p5	AATTGGTAGACACGCTGCTC	AGAATTGAAACGATCGAAAAAGA	50.0	30.4	58.3	55.2	254-261 bp	1.0	46.8