Hotspot

petL-petG

LSC · 65491-65683 · 193 bp

Back to Lagotis

Pi 0.0225

MarkerSeek score 63.40

Primer available yes

Label petL-petG

Feature Metadata

label_name: petL-petG
feature_type: igs
region: LSC
length_bp: 193
variable_sites: 6
pi: 0.0225
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 63.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petL-petG_p1	TGGTCTGAGCAAGATACGAC	CAACTGATCACCACGCCTAT	50.0	50.0	57.1	57.7	311-326 bp	1.0	75.3
petL-petG_p2	TGGTCTGAGCAAGATACGAC	AAGGTCCAACTGATCACCAC	50.0	50.0	57.1	57.4	317-332 bp	1.0	73.1
petL-petG_p3	TTGGTCTGAGCAAGATACGAC	CAACTGATCACCACGCCTAT	47.6	50.0	57.8	57.7	312-327 bp	1.0	72.9
petL-petG_p4	GGTCTGAGCAAGATACGACT	CAACTGATCACCACGCCTAT	50.0	50.0	56.8	57.7	310-325 bp	1.0	72.9
petL-petG_p5	TTGGTCTGAGCAAGATACGAC	AAGGTCCAACTGATCACCAC	47.6	50.0	57.8	57.4	318-333 bp	1.0	70.8