Hotspot

rbcL-accD

LSC · 56226-56863 · 638 bp

Back to Lagotis

Pi 0.0178

MarkerSeek score 58.80

Primer available yes

Label rbcL-accD

Feature Metadata

label_name: rbcL-accD
feature_type: igs
region: LSC
length_bp: 638
variable_sites: 14
pi: 0.0178
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-accD_p1	CCAGTGGATACTTTGGATGTGT	ATAGAGTTTCCTTGCCCCCT	45.5	50.0	58.0	58.0	712-715 bp	1.0	66.7
rbcL-accD_p2	CCAGTGGATACTTTGGATGTGT	CATAGAGTTTCCTTGCCCCC	45.5	55.0	58.0	57.9	713-716 bp	1.0	66.3
rbcL-accD_p3	CCAGTGGATACTTTGGATGTGT	CCATAGAGTTTCCTTGCCCC	45.5	55.0	58.0	57.9	714-717 bp	1.0	66.3
rbcL-accD_p4	CCAGTGGATACTTTGGATGTGT	ATACCTGCGCTCTAACTCCT	45.5	50.0	58.0	57.9	767-770 bp	1.0	66.0
rbcL-accD_p5	CCAGTGGATACTTTGGATGTGT	AGAGTTTCCTTGCCCCCTAT	45.5	50.0	58.0	58.0	713 bp	0.666667	52.2