Hotspot

rps16-trnQ-UUG

LSC · 6271-6720 · 450 bp

Back to Lagotis

Pi 0.0248
MarkerSeek score 59.90
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
450
variable_sites
16
pi
0.0248
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AACGGATCGTGTCCTTCAAG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 540-765 bp 1.0 81.5
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 523-748 bp 1.0 81.1
rps16-trnQ-UUG_p3 CAACGGATCGTGTCCTTCAA GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 541-766 bp 1.0 80.5
rps16-trnQ-UUG_p4 CGGATCGTGTCCTTCAAGTC GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 538-763 bp 1.0 79.2
rps16-trnQ-UUG_p5 GGATCGTGTCCTTCAAGTCG GAGGTTCGAATCCTTCCGTC 55.0 55.0 58.4 58.1 537-762 bp 1.0 79.2