Hotspot

rps2

LSC · 15356-16066 · 711 bp

Back to Lagopsis

Pi 0.00281

MarkerSeek score 60.50

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: LSC
length_bp: 711
variable_sites: 3
pi: 0.00281
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ATCACACAATATCCCCAGGT	TAGTTCAGTTCGAGGGTCCT	45.0	50.0	55.9	57.4	884 bp	1.0	58.4
rps2_p2	ATCACACAATATCCCCAGGT	AGTTCAGTTCGAGGGTCCTC	45.0	55.0	55.9	58.7	883 bp	1.0	57.3
rps2_p3	ATCACACAATATCCCCAGGT	GTCCTCGTCGCTTTGTTTTT	45.0	45.0	55.9	57.2	869 bp	1.0	57.2
rps2_p4	AATCACACAATATCCCCAGGT	TAGTTCAGTTCGAGGGTCCT	42.9	50.0	56.7	57.4	885 bp	1.0	56.8
rps2_p5	AATCACACAATATCCCCAGGT	AGTTCAGTTCGAGGGTCCTC	42.9	55.0	56.7	58.7	884 bp	1.0	55.7