Hotspot

petD

LSC · 75423-76647 · 1225 bp

Back to Lagopsis

Pi 0.00272

MarkerSeek score 60.30

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1225
variable_sites: 5
pi: 0.00272
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	AGTTGAAGGGAATTCTCCGA	AGGGAAAATTCGCTTTGAAGC	45.0	42.9	56.2	57.7	1328 bp	1.0	55.1
petD_p2	TGAAGGGAATTCTCCGAAGAG	AGGGAAAATTCGCTTTGAAGC	47.6	42.9	57.1	57.7	1325 bp	1.0	54.7
petD_p3	AGTTGAAGGGAATTCTCCGAAG	AGGGAAAATTCGCTTTGAAGC	45.5	42.9	58.1	57.7	1328 bp	1.0	53.4
petD_p4	GTTGAAGGGAATTCTCCGAAGA	AGGGAAAATTCGCTTTGAAGC	45.5	42.9	58.1	57.7	1327 bp	1.0	53.4
petD_p5	AGTTGAAGGGAATTCTCCGAA	AGGGAAAATTCGCTTTGAAGC	42.9	42.9	56.9	57.7	1328 bp	1.0	53.3