Hotspot

petB

LSC · 73826-75208 · 1383 bp

Back to Lagopsis

Pi 0.00241

MarkerSeek score 46.80

Primer available yes

Label petB

Feature Metadata

label_name: petB
feature_type: gene
region: LSC
length_bp: 1383
variable_sites: 5
pi: 0.00241
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB_p1	GGTAAAGTAGCTCCCGGTTG	TTCAAGCGAACCAGCCTATC	55.0	50.0	58.0	58.0	1969-1987 bp	1.0	80.8
petB_p2	GTAAAGTAGCTCCCGGTTGG	TTCAAGCGAACCAGCCTATC	55.0	50.0	58.0	58.0	1968-1986 bp	1.0	80.8
petB_p3	GGTAAAGTAGCTCCCGGTTG	CAAGCGAACCAGCCTATCTT	55.0	50.0	58.0	58.0	1967-1985 bp	1.0	80.8
petB_p4	GTAAAGTAGCTCCCGGTTGG	CAAGCGAACCAGCCTATCTT	55.0	50.0	58.0	58.0	1966-1984 bp	1.0	80.8
petB_p5	GTAAAGTAGCTCCCGGTTGG	CTTCAAGCGAACCAGCCTAT	55.0	50.0	58.0	58.0	1969-1987 bp	1.0	80.8