Hotspot

ccsA

SSC · 112377-113339 · 963 bp

Back to Lagopsis

Pi 0.00415

MarkerSeek score 38.20

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 963
variable_sites: 6
pi: 0.00415
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 38.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1683 bp	1.0	80.1
ccsA_p2	TGAAATCGGTAGACACGCTG	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1682 bp	1.0	80.1
ccsA_p3	GTGAAATCGGTAGACACGCT	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	1682 bp	1.0	80.1
ccsA_p4	TGAAATCGGTAGACACGCTG	CAGTAGCTTTTCGATGGCCT	50.0	50.0	58.0	58.0	1681 bp	1.0	80.1
ccsA_p5	ATGTTTTTGGGGGAGGGTTC	TCAGTAGCTTTTCGATGGCC	50.0	50.0	58.0	58.0	1931 bp	0.666667	66.7