Hotspot

ndhG-ndhI

SSC · 116696-117067 · 372 bp

Back to Lagopsis

Pi 0.00896

MarkerSeek score 59.70

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 372
variable_sites: 5
pi: 0.00896
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	TCCTAACGAAAAGGCCGAAT	TCAAATTGCTTTAGGTCGGT	45.0	40.0	57.2	55.3	586-598 bp	1.0	52.6
ndhG-ndhI_p2	TCCTAACGAAAAGGCCGAAT	TCAAATTGCTTTAGGTCGGTT	45.0	38.1	57.2	56.1	586-598 bp	1.0	51.1
ndhG-ndhI_p3	AGACCCGATCCCAGAAATACT	TCAAATTGCTTTAGGTCGGT	47.6	40.0	58.0	55.3	522-534 bp	1.0	50.5
ndhG-ndhI_p4	TCCTAACGAAAAGGCCGAAT	ATCAAATTGCTTTAGGTCGGT	45.0	38.1	57.2	55.8	587-599 bp	1.0	49.3
ndhG-ndhI_p5	AGACCCGATCCCAGAAATACT	TCAAATTGCTTTAGGTCGGTT	47.6	38.1	58.0	56.1	522-534 bp	1.0	49.0