Hotspot

rpl32-trnL-UAG

SSC · 111473-112196 · 724 bp

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Pi 0.006

MarkerSeek score 60.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 724
variable_sites: 6
pi: 0.006
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGCAGCGTTAAAAGCATTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	696-854 bp	1.0	83.9
rpl32-trnL-UAG_p2	GGGCAGCGTTAAAAGCATTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	57.9	58.0	697-855 bp	1.0	83.9
rpl32-trnL-UAG_p3	AAAAAGAAGGGGTATCGGGC	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	712-870 bp	1.0	83.4
rpl32-trnL-UAG_p4	AAAAAGAAGGGGTATCGGGC	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	713-871 bp	1.0	83.4
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCATTT	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	57.9	57.9	706-864 bp	1.0	83.0