Hotspot

rpl20-rps12

LSC · 68048-68809 · 762 bp

Back to Lagopsis

Pi 0.00175

MarkerSeek score 60.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 2
pi: 0.00175
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGAATGAGACCCCCGAAAAC	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	927-928 bp	1.0	77.1
rpl20-rps12_p2	AGACCCCCGAAAACTTGATG	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	921-922 bp	1.0	77.1
rpl20-rps12_p3	ATGAGACCCCCGAAAACTTG	ACAGCCCATTCGAAATGTCA	50.0	45.0	57.8	57.8	924-925 bp	1.0	77.1
rpl20-rps12_p4	TGAATGAGACCCCCGAAAAC	ACAAGACAGCCCATTCGAAA	50.0	45.0	57.8	57.7	932-933 bp	1.0	76.5
rpl20-rps12_p5	AGACCCCCGAAAACTTGATG	ACAAGACAGCCCATTCGAAA	50.0	45.0	57.8	57.7	926-927 bp	1.0	76.5